2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. The spectra analysis and K d were determined using Sigmaplot 14. ZERO BIAS - scores, article reviews, protocol conditions and more. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). . SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. . The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Note: Additional readers with similar performance are listed. Price from $9. View the Molecular Devices website. 2. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Spectramax I3x, supplied by Danaher Inc, used in various techniques. i3x Specifications. SpectraMax i3x Multi-Mode Microplate Reader. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. ATP (“flash” luminescence using . Figure 2-23: Bottle holder To install the bottle holder: 1. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Not compatible with Home Edition. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The dual auto injector module. Published Dec 16, 2020 | Updated Oct 10, 2023. ZERO BIAS - scores, article reviews, protocol conditions and more. Align the two slots on the bottle holder with the two pegs on the rail. User-upgradeable detection modules allow you to expand the capabilities of your microp. SpectraMaxi3Multi. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The user guide also covers the features and specifications of the SpectraMax MiniMax 300 Imaging Cytometer, an optional accessory that. . using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Bioz Stars score: 98/100, based on 1 PubMed citations. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Figure 1. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. This process should take about 3 - 5 minutes. Background Parallax,Columns 2. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Curcumin binding assay. ; The top 3 importers of Spectramax are India with 365. user upgradeable. Figure 1. 7 Figures from 1126 Publications. SpectraMax i3x, onboard luminescence 121. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. ISM Equipment Classification (Group 1, Class A) This equipment is. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). Homogeneous and eterogeneous microplate assays can be performed in one flexible system. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The Results. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 58% for the detection module and 2. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Spectranet customer portalThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. . Amplitude : 1. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. Description 1. My proficiency in techniques such as •PBMC isolation, counting and. . 6% for the on-board optics (monochromators). SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). 306 plate air cal fail,low light. The calculated LOQ values were 0. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. ü; ü. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. 3. 7 mm for all shake intensities. Explore PDF for more details. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Spectranet customer portal. Multimode Readers Comparison Chart. Average 98 stars, based on 1 article reviews. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. SpectraMax i3x Register or Sign In. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. SpectraMax ® iD3 and iD5 Multi-Mode . D 1 1. Register to download your free eBook today. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. Fluorescent Polarization: Solid black plates, as above. Pick the best speed, sensitivity, and price for your needs. SpectraMax i3x Multi-Mode Detection Platform User Guide. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Image analysis provides accurate data. . User-upgradeable detection modules allow you to expand the capabilities of your microp. 2 only) SpectraMax M-series Microplate readers. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. gen) on a Spectramax i3X luminometer. Specifically, we present neurite. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Calcium. BMG LABTECH VANTAstar. Find regional support. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. D 1 1. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. . 0´ 104 4. 314 low lamp level warning. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. i3. SpectraMax® i3x. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. Example: [Setup]. 312 gain calibration failed. The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. It allows you to explore cellular pathways and protein activation and expression in one system. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. See Unpacking the SpectraMax Paradigm Instrument on page 1. No accessories are needed and SoftMax® Pro. 304 signal level saturation. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. We would like to show you a description here but the site won’t allow us. 4. 蛍光 発光 ELISA. More Product Information. 305 reference level saturation. . Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Find regional support. 0. Figure 4. Unparalleled performance on a personalized platform. . 2 Parallel Line Analysis Conc log [ng/ML] RLU. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Monday to Friday 7am - 5pm Pacific Time. . . Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. SeeSpectraMax MiniMaxImagingCytometeronpage85. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax® i3x. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. AlphaScreen. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. The. 3: The cuvette chamber. o For example: Ex: 485/Em 525/Cutoff 515 4. ZERO BIAS - scores, article reviews, protocol conditions and more. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Version 7. Bioz Stars score: 98/100, based on 1 PubMed citations. 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Contact Local Team. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. Future ready The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. The SpectraMax® i3x reader allows you to. . The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. 100 fmol 1536-well (8 µL) 0. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. . Automated for ease of use. Learn more about its features, specifications, and applications in this user guide. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. In this product video, see how the SpectraMax M5 quickly reads samples. SpectraMax i3x Multi-Mode Detection Platform. Unlimited breadth of. Tristar 5 Multimode Microplate Reader. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). As. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. SpectraMax i3x and Paradigm readers require the FP-FLUO. Negative samples contained only MycoAlert Assay Buffer. 2 x 10-14. 5. Each heating element is independently. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. Microplate reader assays requiring infrared (IR) or red-shifted. Biochrom New EX Read 400 Microplate Reader. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Filterless readers provide consistent results with steady temperature regulation. . . In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Citation 2002). Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. 5 decades, with sensitive detection of cells. Get a front row view of your cells. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. . Reporter Assay. g. SpectraMax® L reader support; ü. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Molecular Devices ScanLater Western Blot Detection Platform. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. HTRF IP standard curve. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. Antigen Quantitation Protocol. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. 3. user upgradeable. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Supported Windows Operating System: See release notes. 0001 0. Eliminate time-dependent substrate addition steps. Molecular Devices SpectraMax i3x Multi-Mode Microplate Platform. Open the catalog to page 2. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Download PDF. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. SpectraMax® Microplate Readers and SoftMax® Pro Software. Ease of use. Promega. However, no piece of equipment is immune to breaking down. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. . Cell counters for accurate and. Description. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Examples: “Press carefully to engage,” “ Do not press down. Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. Structured Review. SpectraMax i3x Register or Sign In. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. . Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. 0. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. 50: $75: Improving patient lives by advancing the field of resuscitation medicine. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. 1. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 304 signal level saturation. to View Figures. . MitoXpress-Xtra Oxygen Consumption Assay - Agilent. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Molecular Devices. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Rutin was used to generate standard curves for quantification. Description 1. Examples: “Press carefully to engage,” “ Do not. detection cartridge LUM96: 59. For wavelengths in the UV range above 220 nm,. Explore a wealth of applications with the future-ready SpectraMax i3x. Itemploysa. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. SpectraMax i3xは、吸光・蛍光・発光スペクトル測定のためのマルチモードマイクロプレートリーダーです。. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. Figure 1. 2 or higher,. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. 1. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. It allows you to explore cellular . 312 gain calibration failed. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. Get a front row view of your cells. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). The corrected absorbance is within 5% of the. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 1 and above: Compatible with only with 64-bit OS. 313 reference gain check fail. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. • Removing the transport locks from the PMT shutters and drawers. +44 118 944 8000. no. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 1. Description 1. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Failed to load PDF file. SpectraMax iD3 and iD5 Register or Sign In. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. com. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. . each new assay. Figure 3. MC38 in vivo tumor model in hPD-1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. . ，高内涵成像分析技巧——3D功能展示1-3D预览、共定位、测距，SpectraMax Mini 多功能微孔板读板机带你自信地迈出第一步！ ，多功能酶标仪 SpectraMax i3x 之动力学检测法的设置，iD5注射器卡盒操作视频 - 20201228_8，SpectraMax iD 系列触控屏 overview，【网络讲堂】高内涵. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. Settings not required by a reader are indicated by ‘---’. *On the SpectraMax i3x reader only. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. . GFP expression imaged on the cytometer. We would like to show you a description here but the site won’t allow us. Download PDF. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Unlimited breadth of. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Recommended settings for SpectraMax iD5 and i3x readers. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Note: Additional readers with similar performance are listed. Replicate values were averaged and imported into Design-Expert 8. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax. As.